In recent years, the use of glass fiber-reinforced polymer (GFRP) as an alternative reinforcing material in concrete structures has emerged as an innovative solution to overcome the corrosion problem. In addition to its non-corrodible nature, GFRP reinforcement presents many advantages such as a high strength-to-weight ratio, ease of handling, and immunity against electrochemical corrosion. This paper presents an experimental study to evaluates the behavior of reinforced concrete deep beams, the deep beams provided with glass fiber reinforced polymer (GFRP) with different ratios (0, 0.25%, 0.40%) as web reinforcement configuration rather than traditional steel web reinforcement. All reinforced concrete deep beams have the same span to depth ratio (a/d) 0.40. The shear behavior of the seven experimental concrete deep beams reinforced in shear with GFRP configuration or with/without steel web reinforcement was investigated in this paper. All top and bottom longitudinal reinforcement are steel bars with the same ratios in all tested beams. The deep beam capacity increased by web reinforcement ratio increase. The type of web reinforcement did not affect the number of bending cracks but affected shear cracks. The energy absorption, stiffness of beam, ductility index, and deformability factor decreases by web reinforcement increase. The results are compared with the traditional ACI design method, strut, and tie model to demonstrate the effect of web reinforcement ratio on capacity.