Four simple, precise and sensitive UV- spectrophotometric methods were developed for the first-time determination of tenofovir alafenamide in presence of its alkaline degradate. The first method was a dual wavelength based on determination of the drug at 235.5 nm and 261.5 nm. The second was First – derivative spectrophotometric method in which the amplitude values were measured at 274 nm using Δλ of 8 nm and a scaling factor of 20. The third one was ratio difference depended on measuring the difference in peak amplitudes of tenofovir alafenamide ratio spectra at 261.5 nm and 252 nm using 10 μg mL-1 of its alkaline degradate as a divisor. While the fourth method is a first derivative of ratio spectra measuring the amplitude at 275.6 nm using Δλ = 8 nm and a scaling factor = 10. Beer's law was obeyed in the range of 5-35 μg mL–1 in all methods. The developed methods were successfully applied for the determination of the studied drug in bulk powder and dosage form. All methods were validated according to ICH guidelines and statistically compared to a reported method. Moreover, two assessment tools (Analytical eco-scale and Green Analytical Procedure Index (GAPI) were used to evaluate the greenness of the proposed methods in comparison with the reported one.