Introduction: Energy drinks are widespread drinks targeted to young people as a stimulant to increase mental concentration. Avocado oil is a natural oil extracted from avocado fruit. Aim of the work: This work was performed to study the effect of energy drink on the histological structure of pancreas of adult male albino rat and to evaluate the possible protective role of avocado oil. Materials and Methods: 40 adult male albino rats were divided equally into 4 groups; control group, avocado oil-treated group (0.4 mg/100 gm body weight), energy drink-treated group (1.5 mg/100 gm body weight) and both avocado oil and energy drink treated group. All animals were orally administrated once daily for 4 weeks. Results: Energy drink-treated group revealed focal disturbed architecture of the exocrine parts of pancreas. The exocrine pancreas appeared with cytoplasmic vacuolations and deeply stained nuclei of the acinar cells. Ultrastructural examination showed acinar cells with swollen mitochondria with disrupted cristae, dilated rough endoplasmic reticulum in addition to shrunken condensed nuclei with dilated perinuclear cisternae. In contrast, minimal changes were observed in rats treated concomitantly with avocado oil and energy drink. Conclusion: The concomitant intake of avocado oil could to some extent ameliorate such changes probably through its anti-oxidant and anti-inflammatory effects.