Background: Polycystic ovarian syndrome (PCOs)is one of the most endocrine disorders of females in reproductive age that is accompanied with undesirable metabolic, reproductive, endocrine, and psychological manifestations. The aim of this study is to assess knowledge and self-reported practices of nursing students regarding polycystic ovarian syndrome. Method: A descriptive cross-sectional study design was utilized to accomplish this study. A purposive sample of 420female nursing students in the first level, Faculty of Nursing. Three tools were developed by the researcher for collecting the data of this study as the following: socio-demographic and economic characteristics of nursing students, knowledge, and self-reported practices of nursing students regarding polycystic ovarian syndrome. Results indicated that 87.4% and 89.5% of nursing students had poor knowledge and self-reported practices regarding polycystic ovarian syndrome respectively. The researcher concludes that almost all nursing students have poor level of knowledge and self-reported practices regarding polycystic ovarian syndrome respectively. Finally, the researcher recommended a continuous health education program regarding polycystic ovarian syndrome of nursing students.