A field experiment was carried out in the El-Gemmeiza Agricultural Research Station in the Gharbeya Governorate, Egypt, over the summer seasons of 2022 and 2023. This study examined the effects of different rates of nitrogen (0, 50, 75 and 100 of the recommended rate (RRN) equals 00, 60, 90 and 120 kg N/feddan) in the form of urea and spraying with Ascobein (0 and 200 g/150 liter water/fed.) after one and two month of planting on plant growth, productivity, and grain quality of maize cultivar triple hybrid 320 in clay soil. The interaction between fertilizing maize plants at a rate of 75% RRN and foliar spraying with ascobein at a rate of 200 g/150 liters resulted in an increase in plant height, the weight of 100 grains, the yield of both grain and straw, as well as the biological yield/feddan, in addition to harvest index, also, the concentrations of nitrogen, phosphorus, and potassium as well as their uptake by grains and straw in both seasons as well as net return, and beneficial cost ratio. While, the interaction between 50% RRN the recommended rate, and spraying with ascobein to increase the efficiency of nitrogen use. In order to maximize maize production, we can reduce the recommended amount of nitrogen fertilizer for maize from 120 to 90 kg N/feddan by using spraying with ascobein under the same conditions similar to conducting this research.