Most Streptomyces species are well known to be effective as biological control microorganisms against numerous pathogens; hence, Streptomyces genus is best described as the foundation of all utilized antibiotics on planet earth. Antibiotics are utilized in a variety of contexts, including agriculture and medicine. Authors of this work have effectively isolated Streptomyces species from soil samples gathered across several Egyptian governorates. The antimicrobial activity of Streptomyces isolates against strains of several phytopathogenic fungi, including Alternaria sesame, Fusarium oxysporum, and Rhizoctonia solani, was assessed following collection, isolation, and molecular identification. From all Streptomyces isolates investigated, antifungal assessment results demonstrated that BF2, BF16 and GH11 were the most effective isolates against fungi. In this study, authors utilized transmission electron microscope, traditional morphological and advanced molecular procedures to better characterize Streptomyces' antimicrobial properties. Results proved that isolates, BF2, BF16 and GH11, belong to Streptomyces genus. 16S rRNA nucleotide sequence from BF2, BF16 and GH11 were deposited to the National Center for Biotechnology Information (NCBI) and assigned accession numbers; BF2 (ON130145), BF16 (ON130199) and GH11 (ON130172), respectively. Based on 16S ribosomal Ribonucleic Acid (rRNA) gene sequencing, data revealed that isolates BF2, BF16 and GH11 are affiliated with Streptomyces vietnamensis, S. antibioticus and S. mediolani, respectively. This work supports investigations into novel biocontrol strategies for phytopathogenic species that are just as successful as conventional fungicides. These strategies are believed to generate interest worldwide since they lessen the use of pesticides in agriculture while also having a favorable environmental impact.