425944

Simultaneous quantification of Empagliflozin and Metformin in pharmaceutical dosage form using HPLC technique

Article

Last updated: 11 May 2025

Subjects

-

Tags

Basic and applied research of Chemistry,

Abstract

A reversed-phase high-performance liquid chromatography (HPLC) method was developed and validated for the simultaneous quantification of empagliflozin and metformin in their combined dosage form. The analysis was performed on an Agilent 1200 system equipped with a diode array detector (DAD) using a Hypersil BDS C18 column (4.6 mm x 15 cm, 5 μm). The mobile phase consisted of an isocratic 70:30 (v/v) mixture of phosphate buffer (prepared by dissolving 6.8 g potassium dihydrogen orthophosphate in 1 L water and filtered with a 0.45 μm nylon filter) and acetonitrile. The flow rate was set to 1.5 mL/min, and the injection volume for both empagliflozin and metformin was 10 μL. Detection was performed at 255 nm using the DAD. The method demonstrated linearity over concentration ranges of 3.125-9.38 μg/mL for empagliflozin (retention time: 6.4 min) and 250-750 μg/mL for metformin (retention time: 2.6 min) with correlation coefficients exceeding 0.999. The relative standard deviation (RSD) was less than 2%. These results demonstrate that the method is rapid, accurate, precise, and sensitive, making it suitable for routine quality control of tablets containing both drugs in pharmaceutical settings

DOI

10.21608/bfszu.2025.294373.1396

Keywords

Method validation Empagliflozin, Metformin, HPLC

Authors

First Name

Alaa

Last Name

Amin

MiddleName

-

Affiliation

Department of Chemistry, Faculty of science, Benha University.

Email

asamin2005@hotmail.com

City

-

Orcid

0000-0002-5616-435X

First Name

Soha

Last Name

Mohammed

MiddleName

-

Affiliation

Department of Chemistry, Faculty of science, Zagazig University.

Email

sohafahim@zu.edu.eg

City

-

Orcid

0000-0001-7175-3163

First Name

Mourad

Last Name

Abo-Taleb

MiddleName

-

Affiliation

Department of Chemistry, Faculty of science, Zagazig University.

Email

mm.abotaleb@science.zu.edu.eg

City

-

Orcid

0009-0003-8548-7662

Volume

2025

Article Issue

1

Related Issue

55524

Issue Date

2025-04-01

Receive Date

2024-06-01

Publish Date

2025-04-01

Page Start

76

Page End

85

Print ISSN

1110-1555

Online ISSN

3062-5416

Link

https://bfszu.journals.ekb.eg/article_425944.html

Detail API

http://journals.ekb.eg?_action=service&article_code=425944

Order

41

Type

Original Article

Type Code

838

Publication Type

Journal

Publication Title

Bulletin of Faculty of Science, Zagazig University

Publication Link

https://bfszu.journals.ekb.eg/

MainTitle

Simultaneous quantification of Empagliflozin and Metformin in pharmaceutical dosage form using HPLC technique

Details

Type

Article

Created At

11 May 2025