The maturation of fish gonads in aquaculture containers requires strategic measures so that fish can reproduce. Reproduction of bileh fish in aquaculture containers experiences obstacles, namely slow gonad maturation. Gonad maturation is influenced by internal factors such as the hormonal system and external factors including nutrition. This study aimed to determine the effect of PMSG hormone administration and essential fatty acids on the maturation of female bileh fish gonads. The experimental research design used a complete randomized design, with four treatments and three replicates. The treatment groups were: T1= control, T2= PMSG hormone 1ml/ kg, T3= corn oil 2% + fish oil 1.5%, and T4= PMSG 1ml/ kg + corn oil 2% and fish oil 1.5%. The research stages included preparing materials and tools, mixing treatment ingredients into feed, proximate analysis of feed, fish rearing and treatment, observation, and data collection. The research parameters evaluated were the gonadosomatic index (GSI), hepatosomatic index (HSI), brood weight gain (Wg), gonad maturity level (GML), fecundity, and egg diameter. Data were analyzed using variance analysis and Duncan's test. The results showed that administering PMSG hormone and essential fatty acids through feed significantly affected (P<0.05) IGS, IHS, Wg, and fecundity of bileh fish. The best treatment was T4.