Toxicity of five systemic fungicides on radial growth of Macro‌phomina phaseolina (Tassi) Goid and Cephalophorium gregatum Arlington and Chamberl, the causal pathogens of damping-off and root-root dis‌eases of soybean, were determined. In vitro, results showed that Benlate T, Quinolate CTS and Vitavax SP were the most inhibitory to fungal gro‌wth. In greenhouse, fungicides (Deflate T, Quinolate CTS and Vitavax SP), applied as seed dressing, varied in their effect on nodulation, fresh weight and damping-off of soybean seedlings. Benlate T resulted in the least nodulation and seedling fresh weight and highly affected pre-and post-emergence damping-off of soybean seedlings, while Quinolate CTS and Vitavax SP differed in their effect on bacterial nodulation and seedl‌ings fresh weight and were less effective on damping-off infection. In field experiment, seed dressing with fungicides significantly de-creased damping-off and increased healthy survivals in uninfested plants, which increased seed yield. Studying the translocation of Benlate T, Vitavax SP and Quinolate CTS in soybean seedlings, indicated that three systemic fungicides re‌mained active in stem tissues for at least 30 days.