The present study was carried out to investigate the Effect of Synbiotic Dietary Supplementation on the Lipid Profile and Liver Functions of Hyperlipidemia in Rats. In this study twenty-eight, male albino rats (Sprague-Dawley strain) were divided into four groups as follows: Group 1: as a negative control group and Group 2: as a positive control group. Group 3 was fed a high-fat diet (HFD) for a 7-week experimental period, during which, starting from the third week, the rats were orally administered 1 mL of probiotics containing 1 × 10⁹ CFU/mL twice daily via oral gavage, and dried banana was added to the HFD as a prebiotic at a concentration of 3%.  Group 4: was fed on basal diet mixed with grind dried banana at a concentration of 3% and was given orally one mL of 1 × 10⁹ CFU probiotics / mL twice daily for seven weeks. The effect of synbiotic were studied on lipid profile (TC / TG / LDL-C / HDL-C / VLDL-C) and liver functions (ALT / AST / ALP). Measurements of Initial Body Weight (IBW), Final Body Weight (FBW), Feed Intake (FI), Body Weight Gain (BWG), Feed Efficiency Ratio (FER) in rats was also carried. The results suggest that synbiotics (green banana with bacteria) positively affect lipid metabolism by lowering harmful lipids (VLDL, LDL) and increasing HDL (good cholesterol, which may aid in hyperlipidemia management. While the Control (+Ve) group exhibited the highest feed efficiency, weight gain, and feed intake, the synbiotic groups (G3 and G4) demonstrated a marked reduction in feed efficiency and body weight gain, with G4 notably showing actual weight loss despite higher food intake. This indicates a strong modulatory effect of synbiotics on energy balance and growth performance. Additionally, both groups showed reductions in liver enzyme levels (ALP, ALT, AST) compared to the Control (+Ve), reflecting a protective effect on liver function. Notably, G4 showed the most significant overall improvements in lipid profile, liver enzyme levels, and growth performance, making it the most effective group in reducing liver stress due to consuming high fat diet and improving metabolic health by enhancing blood lipid levels and promoting growth