This study investigated gene effects and epistasis for yield traits in two wheat hybrids: Gemmeiza 11 x YR8 (Cross I) and Misr 2 x YR8 (Cross II), using six population means (P1, P2, F₁, F₂, BC₁, and BC₂). the experiment was conducted at Sakha Agricultural Research Station over the 2019/2020 to 2021/2022 seasons. The Yr8 yellow rust resistance gene was introduced into Egyptian wheat cultivars Gemmeiza-11 and Misr-2 through conventional crossing. Both hybrids with Yr8 showed strong field resistance. High genetic variance and broad-sense heritability estimates for these crosses suggest that effective selection for yellow rust resistance is feasible in the segregating generations. The highest frequencies of resistant F2 plants were found in hybrids combining Yr8 as compared with susceptible cultivars, demonstrating the gene's effectiveness. Thus, incorporating and pyramiding Yr8 into the national wheat breeding program is recommended to improve yellow rust resistance Egyptian wheat. Selected F2 plants provide valuable genetic variation for developing high-yielding, rust-resistant wheat germplasm. SSR marker analysis was pivotal for molecular characterization. Since SSR markers provide the effective in detecting the Yr8 gene due to their high polymorphism. Distinct banding patterns confirmed successful Yr8 gene introgression in parental lines and hybrids. This marker-assisted selection (MAS) approach facilitates early identification of resistant genotypes, enhancing breeding efficiency and accelerating the development of yellow rust-resistant wheat varieties. SSR markers' ability to detect desirable traits at the seedling stage reduces reliance on extensive phenotypic screening, thereby expediting the breeding process.