The kidney, due to its high blood flow, is particularly vulnerable to drug-induced damage and injury. Gentamicin, is a widely used and highly effective antibiotic, moreover it is a main cause of drug-induced renal injury. Therefore, identifying a natural and safe agent to mitigate Gentamicin nephrotoxicity is one of utmost importance. Cystone® is a natural compound possessing antimicrobial and anti-urolithiatic properties, which help preventing stone formation. Bee venom comprises a diverse array of biologically active proteins that pharmacologically mitigates various pathological conditions. Its high-potency compounds exhibit selective and specific activities, rendering them valuable as pharmacological tools in drug research, potential templates for drug design, and therapeutic agents. Bee venom therapy is a highly effective treatment, as it stimulates the release of growth factors from activated platelets and fibroblasts in renal tissue. Specifically, Bee venom therapy up-regulates the gene expression of platelet-derived growth factor, which in turn up-regulates vascular endothelial growth factor, promoting tissue repair and regeneration. Moreover, our findings show that co-administration of gentamicin and Cystone® induces severe kidney damage and stone formation in rats. In contrast, Bee venom injection alleviates this detrimental effect. The present study aims to investigate the modulatory effects of Bee venom on renal tissue regeneration and gentamicin-induced renal damage. Furthermore, we seek to compare the potential protective effects of bee venom and Cystone® on gentamicin-induced renal tissue damage in rats.