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426179

Molecular Identification and Phylogeny of Ec-4 Antigen B-Like Gene of Hydatid Disease Caused by Echinococcus granulosus Isolated from Cattle in Basrah South Iraq

Article

Last updated: 11 May 2025

OverviewSimilar Items

Subjects

-

Tags

Diagnostics

Abstract

Background: Cystic hydatid disease is an important infection that affects a wide range of animal species and humans in many world countries with low-quality control procedures of Echinococcus granulosus in canines, especially dogs. Objective: The current study was conducted to identify and explore the genetic evolution in E. granulosus based on the utilization of Ec-4 antigen B-like (EgAgB) gene as molecular target. Methodology: The study included 67 expected liver samples cysts. These samples were subjected to PCR and partial gene sequencing of the EgAgB gene. Results: The results revealed the presence of these cysts in 16 (23.9%) using morphological identification. The PCR revealed that only 4/16 (25%) of the samples were positive for the gene. The genetic evolution-based phylogenetic tree showed that the current study isolates were nucleotide-similar to isolates from the neighbor country; Turkey. Conclusion: The data of this work showed continued existence of the hydatid disease in cattle in the examined city, still involved in a bovine-dog cycle and so represents a reservoir of the parasite which can apply dangerous health impacts on consumers in the city.

DOI

10.21608/ejmm.2025.375182.1563

Keywords

Cestoda, tapeworms, Parasitic infection

Authors

First Name

Hanady

Last Name

Moker

MiddleName

M.

Affiliation

Clinical Laboratory Sciences Department, University of Basrah, Basrah, Iraq

Email

alihuseeinibsh@gmail.com

City

Basra

Orcid

-

First Name

Huda

Last Name

Farhan

MiddleName

S.

Affiliation

Clinical Laboratory Sciences Department, University of Basrah, Basrah, Iraq

Email

-

City

-

Orcid

-

First Name

Suha

Last Name

Mohammed

MiddleName

H.

Affiliation

Clinical Laboratory Sciences Department, University of Basrah, Basrah, Iraq

Email

-

City

-

Orcid

-

First Name

Qutaiba

Last Name

Qasim

MiddleName

A.

Affiliation

Clinical Laboratory Sciences Department, University of Basrah, Basrah, Iraq

Email

-

City

-

Orcid

-

Volume

34

Article Issue

4

Related Issue

54773

Issue Date

2025-10-01

Receive Date

2025-04-13

Publish Date

2025-10-01

Print ISSN

1110-2179

Online ISSN

2537-0979

Link

https://ejmm.journals.ekb.eg/article_426179.html

Detail API

http://journals.ekb.eg?_action=service&article_code=426179

Order

426,179

Type

New and original researches in the field of Microbiology.

Type Code

2,038

Publication Type

Journal

Publication Title

Egyptian Journal of Medical Microbiology

Publication Link

https://ejmm.journals.ekb.eg/

MainTitle

Molecular Identification and Phylogeny of Ec-4 Antigen B-Like Gene of Hydatid Disease Caused by Echinococcus granulosus Isolated from Cattle in Basrah South Iraq

Details

Type

Article

Created At

11 May 2025