ManageChatDiscover
426636

The Presence of Plasmid-Mediated Quinolone Resistance Genes in Pseudomonas Aeruginosa Strains Collected from Clinical Samples in Al-Al Diwaniyah City

Article

Last updated: 11 May 2025

OverviewSimilar Items

Subjects

-

Tags

Antimicrobial agents

Abstract

Background: The growing challenge of antibiotics resistances caused by Pseudomonas aeruginosa (P. aeruginosa, an important opportunistic pathogen) is one of the global healthcare issues. For epidemiological purpose, the identification of resistance genes is crucial. Objectives: The present study aims to determine the prevalence of PMQR encoding genes (qnr-A, qnr-B) phenotypically and genotypically in P. aeruginosa to determine epidemiological prevalence of these genes in Al-Diwaniyah city. Methodology: The study identified P. aeruginosa isolates from wounds and burns exudates, as well as urine samples from urinary tract infections (UTI) by biochemical characterization and VITEK 2 system. Conventional PCR was used to detect the presence of these genes. Results: Burns had a greater prevalence of P. aeruginosa (67.44%) than wounds (20.93%) and UTIs (11.63%). Antibiotic resistance analysis showed that thoroughly drug-resistant (XDR) bacteria predominated in burn infections (72.4%), wound infections (61%), and UTIs (66.7%). Molecular investigation showed that qnr-B and qnr-A are the most common resistance determinants in clinical isolates (97.91%). Antibiotic resistance patterns in wound, burn, and UTI infections were variable, but qnr-A and qnr-B genes showed that wound and burn cases with positive expressions had significantly increased MDR and XDR resistances. Positive expression of the qnr-A and qnr-b genes was associated with increased antibiotic resistance in all infection types, with substantial differences. Antibiotic susceptibility and gene expression studies for P. aeruginosa showed that resistant isolates have consistently high frequencies of positive gene expressions for these genes, especially for Imipenem and Meropenem. All isolates were Nalidixic acid-resistant and expressed these genes more. Only a few P. aeruginosa isolates are MEROPENEM-resistant intermediates. P. aeruginosa was responsive to all antibiotics except Nalidixic Acid. Our results demonstrate a varied correlation between antibiotic susceptibility and infection type, with statistically significant differences. Conclusion: This study demonstrate a predominance of plasmid-mediated fluoroquinolone resistance genes in the examined isolates. It is advisable to optimize fluoroquinolone utilization to maintain their efficacy against multidrug-resistant bacteria.

DOI

10.21608/ejmm.2025.375976.1567

Keywords

P. aeruginosa, PMQR genes, Antibiotic resistance, qnr-A, qnr-B

Authors

First Name

Alaa

Last Name

Jabbar

MiddleName

H.

Affiliation

Department of Medical Microbiology, College of Medicine, University of Kerbala, Kerbala, Iraq

Email

alaa.hassan@s.uokerbala.edu.iq

City

-

Orcid

-

First Name

Masar

Last Name

AL-Mousawi

MiddleName

-

Affiliation

Department of Medical Microbiology, College of Medicine, University of Kerbala, Kerbala, Iraq

Email

masar.almousawi@uokerbala.edu.iq

City

-

Orcid

0000-0002-5485-3485

First Name

Ali

Last Name

Alyassery

MiddleName

J.

Affiliation

Department of Medical Microbiology, College of Medicine, University of Kerbala, Kerbala, Iraq

Email

ali.jalel@uokerbala.edu.iq

City

-

Orcid

0000-0001-9244-9881

Volume

34

Article Issue

3

Related Issue

53640

Issue Date

2025-07-01

Receive Date

2025-04-15

Publish Date

2025-07-01

Print ISSN

1110-2179

Online ISSN

2537-0979

Link

https://ejmm.journals.ekb.eg/article_426636.html

Detail API

http://journals.ekb.eg?_action=service&article_code=426636

Order

426,636

Type

New and original researches in the field of Microbiology.

Type Code

2,038

Publication Type

Journal

Publication Title

Egyptian Journal of Medical Microbiology

Publication Link

https://ejmm.journals.ekb.eg/

MainTitle

The Presence of Plasmid-Mediated Quinolone Resistance Genes in Pseudomonas Aeruginosa Strains Collected from Clinical Samples in Al-Al Diwaniyah City

Details

Type

Article

Created At

11 May 2025