Obesity, one of the worldwide health problems, it considered increase in fat mass accumulation and body weight gain. The main aim of this study was to find the anti-obesity effect of Purslane (Portulaca oleracea) seeds .Thirty (30) adult male albino rats (Sprague-Dawley strain), were distributed into five main groups (6 rats each) as follows: Group (1) negative or normal group (-Ve): Fed on basal diet only as a control negative. Group (2) positive group (+Ve): were fed on high fat diet (HFD) as a control positive. Group (3): were fed on HFD supplemented with the grinded Purslane seeds 5%. Group (4): were fed on HFD supplemented with the grinded Purslane seeds 10%. Group (5): were fed on HFD supplemented with the grinded Purslane seeds 15%. Results indicated that supplementation with Purslane caused a significant decrease (P<0.05) in body weight of obese rats compared to the control positive group. Moreover, serum glucose, leptin, cholesterol, triglycerides, LDL-c, VLDL-c, urea, uric acid, creatinine, AST, ALT and ALP were significantly decreased, while serum HDL-c was significantly increased.