A field experiment was conducted to study the effect of three isolates of Cyanobacteria individually ((Nostoc punctiforme PQ870057, Nostoc parmelioides PQ864768 and Anabaena cylindrica PQ870058)) and a mixture of them with different ratios of nitrogen concentration(Zero,20,40 and 60 kg N/fed). This experiment was conducted in clay soil located in the center of Tanta, Gharbia Governorate. Cyanobacteria isolates showed. A significant increase in plant height, spike length, spike dry weight, number of spikes, grain yield, straw yield, and biological yield. The findings in unequivocally demonstrated that nitrogen fertilization had a major impact on the weight of grains. As a result, the wheat plant's all wheat wield and its components increased by up to 60 kg N/fed nitrogen fertilizer. The application of nitrogen increases all wheat wield and its components and enhances growth. Regarding the Cyanobacteria effect, notable variations found between the employed treatments. It was established by the data Cyanobacteria mixture produced the highest all wheat wield and its components value.