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426401

Therapeutic potentials of aqueous extract of Piper nigrum whole fruits against tramadol-induced oxidative stress and inflammation in rats

Article

Last updated: 11 May 2025

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Subjects

-

Tags

Biochemistry

Abstract

Tramadol is a widely used opioid, high dose of which has been associated with oxidative stress and provocation of inflammation. This study aims to investigate the effects of aqueous extract of Piper nigrum (black pepper) whole fruits in ameliorating tramadol-induced oxidative stress and inflammation in male Wistar rats. The rats were induced using 60 mg/kg tramadol and then treated with different concentration of black pepper aqueous extract (PNAE); 250, 500, and 1000 mg/kg body weight as well as the reference medication Vitamin C (Vit.C), once daily for 14 days. The parameters related to inflammation and oxidative stress were examined. Secondary metabolite constituents found in the extract include tannins, phenols, flavonoids, saponins, alkaloids and glycosides. Administration of tramadol significantly (p<0.05) increased the level of inflammatory metabolites such as tumor necrosis factor-α and interleukin-1β, this was reversed insignificantly (p>0.05) by the Vit.C and BPAE at 250, 500, and 1000 mg/kg. Tramadol also significantly (p< 0.05) invokes oxidative stress by decreasing the level of reduced glutathione (GSH), superoxide dismutase (SOD), and catalase (CAT), while increasing the level of malondialdehyde (MDA), nitric oxide (NO) in liver and kidney tissues. Vit.C as well as at 250, 500, and 1000 mg/kg PNAE significantly (p< 0.05) reversed the level of MDA, GSH, SOD, CAT, thereby attenuating the effects of tramadol especially at the highest dose. These results revealed that the extract has therapeutic potential against oxidative stress and inflammation thereby attenuating and reversing the deleterious effects of tramadol in a dose dependent manner.

DOI

10.21608/bbj.2025.426401

Keywords

Anti-Inflammatory agents, Nitric oxide, Oxidative Stress, Piper nigrum, tramadol

Authors

First Name

QUADRI

Last Name

NURUDEEN

MiddleName

O.

Affiliation

Biochemistry Unit, Department of Biological Sciences, Al-Hikmah University, Ilorin, Nigeria

Email

quadriolaide@yahoo.com

City

-

Orcid

0000-0001-5261-8679

First Name

Sulyman

Last Name

Abdullahi

MiddleName

-

Affiliation

Biochemistry Unit, Department of Biological Sciences, Al-Hikmah University, Ilorin, Nigeria

Email

sulymanabdullahi@gmail.com

City

-

Orcid

0009-0005-7981-1926

First Name

MANSURAT

Last Name

FALANA

MiddleName

B.

Affiliation

Microbiology Unit, Department of Biological Sciences, Al-Hikmah University, Ilorin, Nigeria.

Email

bolman4ever@yahoo.com

City

-

Orcid

0000-0003-4370-3059

First Name

Muhammed

Last Name

ASINMI

MiddleName

R.

Affiliation

Biochemistry Unit, Department of Biological Sciences, Al-Hikmah University, Ilorin, Nigeria

Email

muhammedrobiu@gmail.com

City

-

Orcid

0000-0003-4886-4687

First Name

Muhammad

Last Name

DIKWA

MiddleName

A.

Affiliation

Department of Microbiology and Biotechnology, Faculty of Science, Federal University, Dutse, Nigeria.

Email

muhammaddikwa5@gmail.com

City

-

Orcid

0009-0002-7819-6683

First Name

Oghenetega

Last Name

Oweh

MiddleName

T.

Affiliation

Department of Medical Biochemistry, Faculty of Basic Medical Sciences, Kaduna State University, Kaduna, Nigeria.

Email

owehoghenetega@gmail.com

City

-

Orcid

0000-0002-0051-9975

First Name

Abdulhafeez

Last Name

Buhari

MiddleName

O.

Affiliation

Department of Public Health, Faculty of Health Sciences, Al-Hikmah University, Ilorin, Nigeria.

Email

buharifolarin@gmail.com

City

-

Orcid

0009-0000-2268-3891

First Name

Adedeji

Last Name

Adelagun

MiddleName

A.

Affiliation

Department of Animal Science, Faculty of Agriculture, University of Ibadan, Ibadan, Nigeria

Email

adedejiadelagun@gmail.com

City

-

Orcid

0009-0000-0353-6175

First Name

Usman

Last Name

OKEME

MiddleName

-

Affiliation

Biochemistry Unit, Department of Chemical Sciences, Summit University, Offa, Nigeria.

Email

usman.okeme@yahoo.com

City

-

Orcid

0000-0002-1476-1965

Volume

3

Article Issue

1

Related Issue

51791

Issue Date

2025-01-01

Receive Date

2025-01-21

Publish Date

2025-01-01

Page Start

228

Page End

239

Print ISSN

2974-4334

Online ISSN

2974-4342

Article File

BBJ_Volume 3_Issue 1_Pages 228-239.pdf

PDF . 637.2kB

Link

https://bbj.journals.ekb.eg/article_426401.html

Detail API

http://journals.ekb.eg?_action=service&article_code=426401

Order

426,401

Type

Research Articles

Type Code

2,845

Publication Type

Journal

Publication Title

Biological and Biomedical Journal

Publication Link

https://bbj.journals.ekb.eg/

MainTitle

Therapeutic potentials of aqueous extract of Piper nigrum whole fruits against tramadol-induced oxidative stress and inflammation in rats

Details

Type

Article

Created At

11 May 2025