A 70 days feeding trial was conducted to investigate the effects of two dietary probiotics; Bacillus subtilis KCTC 2217 or Bacillus licheniformis KCCM 11775 with two prebiotics; mannan oligosaccharide (MOS) or fructooligosaccharide (FOS) on Growth performance, Feed Utilization, economic evaluation, chemical body composition and Digestive Enzymes Activities of Nile tilapia fingerlings. Fish averaging 10.8 ± 0.50 g (mean ± SD) were randomly distributed into five treatments with triplicate tanks. A basal control diet (CON) and four synbiotic diets supplementing B. subtilis + MOS (BSM), B. subtilis + FOS (BSF), B.  Licheniformis + MOS (BLM) and B. licheniformis + FOS (BLF). Results showed that dietary B. subtilis with FOS (BSF) and B. licheniformis with MOS (BLM) give the best weight gain and specific growth rate than other treatments. In general, weight gain and specific growth rate of fish fed all synbiotic diets were higher than fish fed control diet. It could be concluded that dietary B. subtilis with FOS (BSF) and B. licheniformis with MOS (BLM) could have beneficial effects on growth performance and feed utilization   in Nile tilapia (Oreochrmis nilticus) fingerlings. These probiotics improved the fish enzyme activities of amylase in the gastrointestinal tract than the fish fed control diet; however, the protease and lipase activities were not affected. The present results recommend the incorporation of probiotics to Nile tilapia feed as supplements to stimulate fish growth and digestion.