Recently, nanoparticles have emerged as the most significant pollutant in the aquatic environment. Aluminum nanoparticles, often known as Al-NPs, are among the metal nanoparticles that are utilized most frequently. The widespread use of aluminum nanoparticles (Al-NPs) results in the contamination of aquatic bodies and the subsequent adverse effects on fish. This work investigated the effects of Al-NPs on the survival rate, external morphology, and gastrointestinal abnormalities of developing zebrafish (Danio rerio) larvae. There were four equal groupings of zebrafish eggs. No therapies were administered to the first group, which served as a control. From the first day after fertilization (dpf) until the fourteenth dpf, the second, third, and fourth groups were subjected to Al-NPs at doses of 40, 80, and 160 mg/L, respectively. In a dose-dependent way, the groups that were exposed to AL-NPs exhibited the highest mortality rates (10%, 50%, and 75% in Al-NPs (Low: L, Medium: M and High: H respectively), external morphological defects, and gastrointestinal malformations  . The percentage of external abnormalities was 4.1 ± 0.28, 25.30 ± 0.10, 38.50 ± 0.18, and 49.8 ± 0.29 in control, Al-NPs (L), Al-NPs (M) and Al-NPs (H) groups respectively. While the percentage of embryos with malformed GIT was 5.5 ± 0.08, 28.20 ±0 .20, 41.20 ± 0.11, and 80.3 ± 0.33 in the control, Al-NPs (L), AL-NPs (M) and AL-NPs (H) groups, respectively. Al-NPs also increased the lipid    percentage of lipid accumulation which was 10 ± 0.01, 50.50 ± 0.07, 65.3 ± 0.23 and 85 ± 0.31 in control, Al-NPs (L), Al-NPs (M) and Al-NPs (H) groups respectively after the transition from larval to juvenile stage. This suggests that Al-NPs have the potential to adversely disrupt the normal development of zebrafish larvae.