Background:  This research was designed to show evaluation of antibacterial activities of Chromolaena odorata (C. odorata) Linn extracts on bacteria associated with wound infections. Methods: A total of 127 wound exudate samples were collected from the patients with chronic wound infections attending the Federal Medical Centre, Owo, First Mercy Hospital and University of Medical Teaching Hospital, Akure, Ondo State, Nigeria, between November 2021 and January 2022. Questionnaires were served to each patient in order to obtain information about the wound. Morphological and biochemical characterization of bacteria were determined using standard microbiological methods. The C. odorata extract was subjected to Soxhlet and maceration extraction methods. The solvents used were cold water, hot water, N-hexane and ethanol. Phytochemical evaluation was carried out on the extracts. The antibiotic susceptibility patterns of the isolates were determined by testing against antibiotics and extracts. Qualitative phytochemical analysis showed the presence of alkaloid, flavonoid, glycoside, phenol, phlobatannin, saponin, steroid, tannin, and terpenoid in the extracts of C. odorata. Results: A total of 13 bioactive compounds were observed in the ethanol extract of C. odorata. The highest leakages of Na+ and K+ were observed in Proteus mirabilis at 12.9 mol/kg and 30.0 mol/kg. There was higher leakages of Na+ in all the test isolates than K+. Conclusions: The antibacterial efficacy of leaf extracts of C. odorata has been evidently proven against bacterial isolates associated with wound infections, suggestive of good alternative treatment materials.