Abstract: honey and natural plants are used for treatment of infections by bacteria as Klebsiella, Pseudomonas, Acinetobacter, Enterobacter and Escherichia coli. The objective of this study was to investigate the antimicrobial activities of honey and some natural plants as garlic, onion, Portulaca oleracea (rigla) against MRSA and some Gram- negative bacteria, to compare their potency with commercially used antibiotics. Methods: In study 60 clinical specimens were collected from hospitalized patients with gastrointestinal, wound and urinary tract infections. All isolates were identified using microbiological standard procedures. Antimicrobial activity of honey, garlic, onion and rigla plant was investigated at concentrations of 100%, 50%, 25%, and 12.5% by measurement of inhibition zones. The antimicrobial activity of mixture of natural plants extracts and commercial antibiotics was also investigated. Results: At a 12.5% concentration, garlic showed inhibition zones of 12 mm and 20 mm against Klebsiella and Enterobacter, respectively, while Citrus honey was more effective on Pseudomonas and Acinetobacter with an inhibition zone of 10 mm. Mixture of commercial antibiotics and plant extracts at concentrations was more effective on Enterobacter. Onion at 12.5% concentration with ceftazidime showed 10 mm inhibition zone, while against Pseudomonas the inhibition zone was 12 mm by clover honey at 12.5% concentration with ceftazidime. Mixture of ethanol extract at 12.5% with ceftazidime was active against Acinetobacter with12 mm inhibition zone. Conclusion: importance of study is identification of multidrug resistance bacteria in human patient at hospitals. Using natural extracts of plants and honeys are safe, efficient and low cost for treatment resistant bacteria.