Background: Surgical site infection (SSI) is a major source of morbidity and mortality. It happens within 30 days of a surgical intervention around or at the incision site. Deeper underlying tissues may be involved. In recent years, multidrug-resistant (MDR) organisms are responsible for a growing proportion of illnesses, which present a significant therapeutic challenge. Aim: This study aimed to evaluate the antibiotic susceptibility pattern of Enterobacteriaceae isolated from SSI in Benha University Hospital and detect the blaNDM-1 gene in carbapenem resistant isolates. Methods: A total of 130 wound samples were cultured on blood agar and MacConkey agar media. Strains were identified by standard bacteriological methods and antibiotic sensitivity testing by VITEK 2 compact system, Detection of MBL (metallo-beta-lactamase) producing isolates phenotypically by double disk synergy test (DDST) and detection of blaNDM-1gene in the resistant strains by Conventional PCR. Results: of 120 culture positive wound samples, 53 strains were Enterobacteriaceae (22Klebsiella species, 20 E.coli, 8 Proteus spp and 3 Citrobacter spp), 59% and 30% of Klebsiella spp and E-coli strains respectively were carbapenem resistant, while none of Proteus or Citrobacter spp were carbapenem resistant. Regarding blaNDM-1 detection it was 61.54% and 66.67% for Klebsiella spp and E.coli respectively. Conclusion: NDM-1 gene propagation in Enterobacteriaceae is a worrying threat which necessitates several control measures.