Background:  Individuals with burn injuries (BI) are particularly vulnerable to infections. Microbial infection led to morbidity and mortality.  This study explores the frequencies of bacteria associated with BI-infection and detects which one is the highly prevalent species. Also, it detects the microbial activity of three kinds of antimicrobial agents involved: alcohol clove extract. TiO2 nanoparticles; and clove extract mediated by TiO2NP. This study enrolled seventy swabs collected from BI-patients.  Methods: We used routine methods and the VITECK assay to detect bacteria. The dilution microplate assay was used to detect the minimum inhibitory concentration (MIC) for all agents. Results of study revealed that VMRSA had a high frequency score of 42.9%, followed by MARSA and pseudomonas aeruginosa at 14.3%. According to microbial activity, clove extract was revealed to have the lowest concentration inhibition growth bacterium at 0.75 mg/ml; additionally, concentration 1.55 mg/ml for TiO2NPs had been virtue inhibition growth bacteria; and also, it had the lowest dilution used as MIC. Furthermore, at a concentration of 0.75 mg/ml, clove extract mediated by TiO2NPs has a significant microbial activity to inhibit the growth of VMRSA more than a single agent microbial. Conclusion: Our finding found that VMRSA is a high-frequency bacterium and also a common bacteria associated with infection-burn injuries in patients. Finally, the clove extract mediated by TiO2NPs at a concentration of 0.75 mg/ml can be considered the MIC for VMRSA. Also, it can be suggested as a potential application in medical felids due to their forceful antimicrobial activity.