Background: Staff nurses must be adequately aware about the importance of entrepreneurial leadership that directly influence on their team climate and innovative work behavior. Aim: To assess the effect of entrepreneurial leadership on team climate and innovative work behavior among staff nurses. Design: A descriptive correlational design. Setting: The study conducted at Main Sohag University Hospital. Subjects: A convenient sample of staff nurses (n=150) working at pre-mentioned setting. Tools: Three tools used for data collection. Tool I consists of two parts namely, personal characteristics form and entrepreneurial leadership questionnaire. Tool II is team climate questionnaire and Tool III is innovative work behavior questionnaire. Results: More than half of staff nurses agreed that leaders have high level of entrepreneurial leadership, more than one third of staff nurses have a high level of team climate and innovate work behavior. Conclusion: More than half of nurse leaders had a high level of entrepreneurial leadership at Sohag University Hospital, more than one third of nurse staff had high level of team climate, also more than one third of them have high level of innovate work behavior. In addition there was highly statistically significant relation between entrepreneurial leadership, team climate, and innovative work behavior as reported by staff nurses. Recommendations: Implement a management strategy that addresses importance of adopting entrepreneurial leadership behaviors for nurse leaders. A reward system should be made to the staff nurses who exhibit innovative work behaviors. Creating encouragement environment for the staff nurses to promote their performance and create positive team climate.