The aim of this study was to assess the adhesion for four different classes of endodontic root canal sealers (AH Plus, Sealapex, TotalFill BC and GuttaFlow Bioseal) to the root dentin by evaluating their push out bond strength (POBS). Material and methods: This study was conducted on 48 extracted human mandibular premolars with single root and single canal. After decoronatation and preparation of the canals with ProTaper files up to X 4, the samples were divided into four groups (n=12) according to the sealer used: AH Plus, Sealapex, TotalFill BC and GuttaFlow Bioseal sealers. The samples were obturated by utilizing the lateral compaction technique and kept in an incubator for one week. The roots were set in self-curing acrylic resin and sectioned transversely to obtain 2 mm-thick slices from the apical, middle, and coronal thirds. The interfacial bonding strength was evaluated by POBS test conducted on a universal testing machine in apical to coronal direction. The results were statistically evaluated using a one-way ANOVA and post hoc Tukey analysis, with a significance level set at p < 0.05. Results: AH plus sealer exhibits a considerably higher POBS in the apical, middle and coronal thirds than other sealers. Sealapex, total fill, and Gutta flow bioseal do not exhibit any statistically significant differences. Conclusions: AH plus sealer demonstrated a higher POBS in comparison to other sealers (Seal apex, total fill, Gutta flow bioseal). The POBS to root dentin was not significantly different between Sealapex, total fill, and Gutta flow bioseal.