A series of in vitro experiments were conducted during the 2022/2023 season at the Tissue Culture Laboratory, Horticulture Research Institute, Agriculture Research Center, Giza, Egypt. The aim was to develop an efficient micro-propagation protocol for Cordyline fruticosa ‘Atom' while maintaining high quality and productivity. The experiments tested different cytokinin types and concentrations with or without adding NAA (for the first multiplication experiment), varying medium salt strengths and explant support methods (for the second multiplication experiment) during the multiplication stage. Also, different medium salt strengths, IBA concentrations, and explant support methods were studied during the rooting stage. For acclimatization, various potting mixtures were used. The results showed that using BA at 3.0 mg/l without NAA for the first multiplication experiment and full or ¾-strength MS medium with a solid medium for the second multiplication experiment yielded the highest values for shoot formation percentage, number of shoots per explant, shoot length, number of leaves per shoot, and pigment content. For rooting, a ¾-strength medium combined with 1.0 mg/l IBA and a solid medium was most effective. Coco peat alone or mixed with sand was the best potting mixture for acclimatizing. The study found BA to be more effective than kinetin, diluted strength medium could be used without reducing productivity or quality, there was no need for NAA during the multiplication stage, low concentrations of IBA were important for rooting, the solid medium outperformed liquid medium (supported with filter paper bridge) and coco peat surpassed peat moss based mixtures.