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425594

Decontamination of Artificially Infectious Urinary Catheter using Gamma Irradiation Technique

Article

Last updated: 04 May 2025

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Subjects

-

Tags

prevention of hospital infections

Abstract

Background: Decontaminating urinary catheters prior to disposal or potential reuse is essential for mitigating environmental hazards and reducing the spread of multidrug-resistant (MDR) pathogens. Objective: This study aimed to evaluate the efficacy of gamma irradiation in decontaminating urinary catheters by inactivating MDR uropathogenic bacteria and disrupting their associated biofilms. Methodology: Fifty strains bacteria were isolated from urine samples obtained from catheterized patients and four bacterial isolates were recorded with 100% resistance against all tested antibiotics. Among them, two isolates were selected and identified as strong biofilm producers, Proteus mirabilis and Staphylococcus haemolyticus. The effect of different doses of Gamma radiation on the viability of the drug resistant strains and biofilm formation were studied. The gamma irradiation technique was applied to decontaminate artificially infectious urinary catheters. Results: The study showed that a dose-dependent reduction in bacterial survival and biofilm formation was observed. The lethal gamma doses for P. mirabilis and S. haemolyticus were 0.5 kGy and 1.5 kGy, respectively. Conclusion: Gamma irradiation is an effective strategy for decontaminating urinary catheters and eliminating MDR uropathogenic bacteria, cause biofilm degradation and offering a potential eco-friendly solution for managing infectious medical waste.

DOI

10.21608/ejmm.2025.376867.1572

Keywords

D10 value, Proteus mirabilis, Staphylococcus haemolyticus, Biofilm

Authors

First Name

Aml

Last Name

Omara

MiddleName

A.

Affiliation

Botany and Microbiology Department, Faculty of Science, Zagazig University, Zagazig, Egypt.

Email

a.abdelraouf@science.zu.edu.eg

City

-

Orcid

-

First Name

Yassmeen

Last Name

Hasanien

MiddleName

A.

Affiliation

Plant Research Department, Nuclear Research Center, Egyptian Atomic Energy Authority, Cairo, Egypt.

Email

yasmeen21485@gmail.com

City

-

Orcid

0000-0002-4368-4903

First Name

Wesam

Last Name

Hassanein

MiddleName

A.

Affiliation

Botany and Microbiology Department, Faculty of Science, Zagazig University, Zagazig, Egypt.

Email

wesamtarek@zu.edu.eg

City

-

Orcid

-

First Name

Fifi

Last Name

Reda

MiddleName

M.

Affiliation

Botany and Microbiology Department, Faculty of Science, Zagazig University, Zagazig, Egypt.

Email

fifireda@zu.edu.eg

City

-

Orcid

-

First Name

Mona

Last Name

Ibraheim

MiddleName

H.

Affiliation

Physics Department, Faculty of Science, Zagazig University, Zagazig, Egypt.

Email

monamekky70@gmail.com

City

-

Orcid

-

First Name

ٍSafaa

Last Name

Abdel-Karim

MiddleName

A.M.

Affiliation

Microbiology and Immunology Department, Faculty of Pharmacy-Zagazig University.

Email

safaaabdelaal85@gmail.com

City

-

Orcid

-

First Name

Hatem

Last Name

Tokhy

MiddleName

H.

Affiliation

Radiation Protection and Dosimetry Department, National Center for Radiation Research and Technology, Egyptian Atomic Energy Authority, Cairo, Egypt.

Email

drhatemhussny2002@gmail.com

City

-

Orcid

-

Volume

34

Article Issue

4

Related Issue

54773

Issue Date

2025-10-01

Receive Date

2025-04-21

Publish Date

2025-10-01

Print ISSN

1110-2179

Online ISSN

2537-0979

Link

https://ejmm.journals.ekb.eg/article_425594.html

Detail API

http://journals.ekb.eg?_action=service&article_code=425594

Order

425,594

Type

New and original researches in the field of Microbiology.

Type Code

2,038

Publication Type

Journal

Publication Title

Egyptian Journal of Medical Microbiology

Publication Link

https://ejmm.journals.ekb.eg/

MainTitle

Decontamination of Artificially Infectious Urinary Catheter using Gamma Irradiation Technique

Details

Type

Article

Created At

04 May 2025