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Soluble Programmed Cell Death Protein-1(sPD-1) as an Early Diagnostic and Prognostic Marker for Sepsis Following Respiratory Tract Infections

Article

Last updated: 04 May 2025

OverviewSimilar Items

Subjects

-

Tags

Clinical microbiology

Abstract

Background: Sepsis is a life-threatening organ dysfunction caused by a dysregulated host response to infection. Soluble Programmed cell death protein-1(sPD-1) increased in patients with sepsis and involved in immunosuppression. It is associated with a poor prognosis, severity and 28-day mortality. Objectives: Identification of the caustive agents of sepsis using Bact Alert 3D system, measurement of serum level of sPD-1 in patients with sepsis compared to control group by ELISA and evaluation of sPD-1 as a diagnostic and prognostic marker for the severity and 28-day mortality. Methodology: Our study was done on 40 patients diagnosed as sepsis in Chest and Intensive Care Units Departments of Benha University Hospital. Blood samples were withdrawn from the patients for blood culture, Subcultures and biochemical tests were done for identification of the caustive agents of sepsis and ELISA is used for for measurment of sPD-1 level in patients and control group. Results: According to the causative pathogens, Klebsiella pneumoniae was the most frequently isolated organism (32.5%), followed by Pseudomonas aeruginosa (22.5%) and Staphylococcus aureus (17.5%). According to sPD-1, the mean serum level was significantly higher in the case group (17.58 ± 15.05 pg/mL) compared to the control group (0.53 ± 0.37 pg/mL). SPD-1 levels were markedly higher in non-survivors compared to survivors. Conclusion: sepsis is a common disease with multimicrobial causes. Klebsiella pneumoniae is the main causative bacteria for sepsis. sPD-1 is a promising diagnostic marker for sepsis with high sensitivity (97.5%), and specificity (95.0%), and prognostic marker for the severity and 28-day mortality

DOI

10.21608/ejmm.2025.376789.1574

Keywords

Sepsis, sPD-1, ELISA, K.pneumoniae, Bact Alert 3D system

Authors

First Name

Rasha

Last Name

ElSayed

MiddleName

A.

Affiliation

Medical Microbiology and Immunology Department, Faculty of Medicine, Benha University, Egypt

Email

rashaabdelhamid43@gmail.com

City

بنها

Orcid

-

First Name

Ahmed

Last Name

Osman

MiddleName

S.

Affiliation

Medical Microbiology and Immunology Department, Faculty of Medicine, Benha University, Egypt

Email

prof.dr.ahmedsaied2020@gmail.com

City

benha

Orcid

-

First Name

Aghadear

Last Name

Mohammed

MiddleName

A.H.

Affiliation

Medical Microbiology and Immunology Department, Faculty of Medicine, Benha University, Egypt

Email

aghadear.mohammed@must.edu.eg

City

qaluib

Orcid

-

First Name

Nashwa

Last Name

Mohammad

MiddleName

A.

Affiliation

Chest and ICU Departments of Benha University Hospital

Email

aminnashwa24@gmail.com

City

-

Orcid

-

First Name

Amira

Last Name

Ramadan

MiddleName

E.

Affiliation

Medical Microbiology and Immunology Department, Faculty of Medicine, Benha University, Egypt

Email

amera.ramadan@fmed.bu.edu.eg

City

-

Orcid

-

Volume

34

Article Issue

4

Related Issue

54773

Issue Date

2025-10-01

Receive Date

2025-04-19

Publish Date

2025-10-01

Print ISSN

1110-2179

Online ISSN

2537-0979

Link

https://ejmm.journals.ekb.eg/article_425215.html

Detail API

http://journals.ekb.eg?_action=service&article_code=425215

Order

425,215

Type

New and original researches in the field of Microbiology.

Type Code

2,038

Publication Type

Journal

Publication Title

Egyptian Journal of Medical Microbiology

Publication Link

https://ejmm.journals.ekb.eg/

MainTitle

Soluble Programmed Cell Death Protein-1(sPD-1) as an Early Diagnostic and Prognostic Marker for Sepsis Following Respiratory Tract Infections

Details

Type

Article

Created At

04 May 2025