Aim: Evaluate Effect of blood contamination on marginal adaptation, surface hardness, and bond strength of two retrograde materials.
Materials and methods: 144 single-rooted mandibular premolars were collected, disinfected, crowns were removed, roots were endodontically treated, and root-end resection was made by cutting 3 mm from apex. Root-end cavities were prepared, then classified into two groups (n=72) according to root-end filling materials, Group I, restored with ProRoot MTA (PMTA), Group 2 restored by Well-Root PT (WRPT). Each group was classified into two subgroups (n=36) according to the setting environment; subgroup A; the materials allowed to set in deionized water (PMTA/W and WRPT/W), while subgroup B; the materials allowed to set in human blood (PMTA/B and WRPT/B). Twelve samples from each subgroup were used to study the marginal adaptation, surface hardness, and bond strength.
Results: In contact to deionized water and human blood, WRPT showed less significant marginal gap distance and non-significant less hardness value than PMTA, the push-out bond strength for WRPT was highly significant than PMTA in contact to deionized water, and highly non-significant than PMTA in contact to human blood. The blood contamination had negative significant effect on marginal adaptation of PMTA, hardness number of WRPT, and push-out bond strength of both root-end filling materials.
Conclusion: The marginal adaptation and bond strength for WRPT are superior to those for PMTA. Blood contamination negatively effect on the studied properties of both materials.