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425415

Determining the Optimal Conditions for Collagenase Production by a Local Isolate of Entrobacter Cloacae Using Local Organic Waste

Article

Last updated: 04 May 2025

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Subjects

-

Tags

Antimicrobial agents

Abstract

Background: Collagenases are proteolytic enzymes with applications in leather processing, food industries, and biomedicine. Utilizing locally sourced organic wastes as fermentation substrates offers a cost-effective and sustainable method for enzyme production. Aim: To identify the optimal physicochemical and nutritional conditions for maximal collagenase production by a native Enterobacter cloacae isolate using organic waste substrates. Methodology: Forty-eight bacterial isolates from soil, animal, and poultry wastes were screened on gelatin and collagen media to select six active strains, then narrowed to three based on performance on various organic wastes. The two highest producers were identified via cultural, microscopic, biochemical tests and the Compact VITEK2 system. One-factor-at-a-time experiments optimized pH (5.0–10.0), temperature (25–45 °C), substrate type (e.g., chicken foot waste), inoculum size (0.5–2.0 ml/100 ml), inoculum age (18–30 h), nitrogen source (peptone, yeast extract, ammonium salts), incubation mode (static vs. shaking), and incubation time (24–72 h). Collagenase activity was quantified in U/ml. Results: Both isolates were confirmed as E. cloacae. Maximum enzyme yield occurred at pH 8.0 and 35 °C using chicken foot waste. The optimal inoculum was 1 ml per 100 ml medium (1.1×10⁹ cells/ml, 24 h age). Peptone under static incubation yielded the highest activity, reaching 20.232 U/ml after 48 h. Conclusion: A local E. cloacae isolate efficiently produces collagenase on chicken foot waste under optimized conditions, demonstrating a scalable, low-cost, and eco-friendly strategy for industrial enzyme production.

DOI

10.21608/ejmm.2025.376361.1568

Keywords

Metalloprotease group, collagenase, chicken feet, Collagen-degrading bacteria

Authors

First Name

Ayoub

Last Name

Nassef

MiddleName

S.

Affiliation

Biology Department, College of Education for Pure Sciences, University of Anbar, Anbar, Iraq

Email

ayo22u1006@uoanbar.edu.iq

City

-

Orcid

-

First Name

Dhafer

Last Name

Al-Rawi

MiddleName

F.

Affiliation

Biology Department, College of Education for Pure Sciences, University of Anbar, Anbar, Iraq

Email

prof.daffer@uoanbar.edu.iq

City

-

Orcid

-

Volume

34

Article Issue

3

Related Issue

53640

Issue Date

2025-07-01

Receive Date

2025-04-17

Publish Date

2025-07-01

Print ISSN

1110-2179

Online ISSN

2537-0979

Link

https://ejmm.journals.ekb.eg/article_425415.html

Detail API

http://journals.ekb.eg?_action=service&article_code=425415

Order

425,415

Type

New and original researches in the field of Microbiology.

Type Code

2,038

Publication Type

Journal

Publication Title

Egyptian Journal of Medical Microbiology

Publication Link

https://ejmm.journals.ekb.eg/

MainTitle

Determining the Optimal Conditions for Collagenase Production by a Local Isolate of Entrobacter Cloacae Using Local Organic Waste

Details

Type

Article

Created At

04 May 2025