Background Sprouted seeds are rich in bioactive substances including unsaturated fatty acids, polyphenols and flavonoids, which have a good influence on stomach health due to their anti-inflammatory and antioxidant properties. Methods The chemical composition, fatty acids, polyphenols and antioxidant activity were estimated. Thirty-five rat were divided into the main group (5 rat), which was the control (ve-), and five subgroups. Four groups were fed the basal diet including (5, 10%) flax sprouts and (5, 10%) broccoli sprouts as a pre-treatment for 7 days. Then, all groups were given ibuprofen (400 mg/kg, orally) twice a day after fasting for 24 hours, the final group was given ranitidine. Ulcer index, gastric juice volume, gastric juice pH, and protection index were measured. Biochemical indicators such as lipid peroxidation, superoxide dismutase (SOD), catalase (CAT), and malondialdehyde (MDA) were evaluated. Results: The results showed significant differences in nutritional composition, fatty acids, polyphenols, and antioxidant activity (DPPH and FRAP). Sprouting was found to have significant nutritional value in both flaxseed and broccoli seeds. Fatty acids varied between increased palmitic and oleic acid contents after germination and decreased stearic acid content in both flax and broccoli sprouts. Broccoli sprouts exhibited higher potency reducing activities compared to flax sprouts. Antiulcer activity was almost identical to that of the positive control. Sprouted broccoli seeds (10%) demonstrated strong antiulcer activity across all macroscopic and biochemical parameters tested.