In this research, a comprehensive seasonal analysis was conducted on various hematological parameters, including aspartate aminotransferase (AST), alanine aminotransferase (ALT), alkaline phosphatase (ALP), and lactate dehydrogenase (LDH), alongside fluctuations in calcium, cholesterol, total protein, triglycerides, creatinine, and phosphorus levels in six-year-old Persian sturgeon (Acipenser persicus) with an average weight of 5000 ± 350 grams. Thirteen specimens were collected at the conclusion of each season, with blood samples obtained from the caudal peduncle and subsequently transported to the laboratory under ice-cold conditions for analysis. The results revealed that the spring season exhibited the highest enzyme activities for ALP (179.38 ± 39.6 IU/L) and LDH (643.1 ± 236.53 IU/L). In contrast, the autumn season recorded peak levels of AST (174.41 ± 35.01 IU/L) and ALT (3.46 ± 3.58 IU/L), while winter showed elevated concentrations of creatinine (0.23 ± 0.03 mg/dL), total protein (4.91 ± 0.55 mg/dL), and phosphorus ions (14.24 ± 1.41 mg/dL), all of which were statistically significant when compared to other seasons (p<0.05). Furthermore, winter also demonstrated significant differences in cholesterol (126.19 ± 34.33 mg/dL) and triglyceride levels (385.50 ± 134.03 mg/dL) relative to other seasons (p<0.05). These findings are essential for establishing baseline blood parameter ranges that can aid in the effective management of breeding practices and the monitoring of the physiological health of this native species.