New hybrids, generally, need to be tested at several locations before being recommended for release. The main objective of this study was the selection for the stability among genotypes of trial B to access genotypes reliable to cultivate in different environment in Middle and Upper Egypt area. Twenty long staple Egyptian cotton genotypes were evaluated at four environments, i.e. Sids, El-Fayoum, Assiut and Sohag in 2016 season as yield trials of a cotton breeding program. Results showed that the variance of components of environments, genotypes, and environments by genotypes interaction were significant (p<0.01) for lint cotton yield. The E component was always the most important source of yield variation. When GGE model was fitted, the first two PCs explained 79.77% (PC1=63.04% and PC2=16.73%) of GGE variation for lint cotton yield. Genotypes ((G 91 × G 90) × {(G83 × G80)× Dandara}), ((G 91 × G 90) × {(G83 × G80)× G89}) and ((G 91 × G 90) × G80) with means over the two control genotypes (Giza 90 and Giza 95) plus the promising hybrid ({(G83 × G80)× G89}×Australian ). Genotypes, ((G 91×G 90) × {(G83×G80) × Dandara}) and ((G 91×G 90)×G80) was identified as high yielder and stable genotype. Three genotypes were the winning genotypes for respective environments, ((G 91 × G 90) × G80) was adaptable in both Sids and Sohag region, (CB 58×G90) adaptable for El-Fayoum region, while Assiut region were ((G 91×G 90)×{(G83×G80)×G89}),((G 91 × G 90) × {(G83 × G80) × Dandara}) and ({(G83 ×G80)× G89} ×Karashinky). Positive correlations between Sids and Sohag region reveals that similar information about the genotypes. The fiber quality of genotypes under study they were in the same category for long staple cotton in Middle and Upper Egypt.