Cancer is a major health problem worldwide and is one of the leading causes of death. Preventing tumor development is a significant challenge due to the similarity in cellular mechanisms between cancerous and normal cells; however, biological substances have high efficiency against cancerous cells and low toxicity against normal cells associated with a good prognosis. Therefore, the current study is designed to study the role of Anise extract (A.E.) and Anise extract- Chitosan nanoparticles in the synergistic effect with Cisplatin in the treatment of tumor-induced in female mice. Materials and methods: Five groups of female mice, six mice for each group, were included to achieve the goal of the present study. These study groups were control, tumor-induced, Cisplatin treated, Cisplatin – Anise extract treated, and Cisplatin- Anise extract- Chitosan nanoparticles treated groups. The tumor size and some biomarkers (IL-33, LRG-1, and CRP) were considered indicators for tumor development and response to the treatment protocol. Results: The present study found that the tumor size in the tumor-induced group reached 33.1 mm after 30 days of inducement, while the tumor size in the treated group was significantly smaller than in the tumor-induced group. The tumor size in the Cisplatin- Anise extract group was smaller than the Cisplatin treated group. Also, the effect of Cisplatin against a tumor increased when combining anise extract with Chitosan nanoparticles. The biomarkers showed that IL-33, LRG-1, and CRP levels were higher in the tumor group than in the control and other study groups. Conclusion: The current study concluded that the anise extract has high effectiveness in reinforcing Cisplatin action against tumor development and, simultaneously, increased their effect by nanoparticles.