In recent years, the use of vermicompost as organic fertilizer which produced by earthworms has become increasingly popular in many fields that could inhibit most fungal diseases. In this study, the effect of seven treatments were conducted in strawberry nursery, Actinomycetes, Trichoderma, bioactive vermicompost, bioactive vermi-wash, chitosan in addition two chemical treatments (potassium permanganate and potassium phosphite) compared to a control one. Ridomil Gold Plus fungicide is used to control charcoal rot caused by Macrophomina phaseolina. Through this study, the treatment of Trichoderma and Actinomycetes in concentration 3L/feddan  extracted from bio active vermicompost has the best results (61.2 and 54.7 %) respectively compared with the other biocontrol treatments, Chitosan (52.2%), Bio active vermi-wash (50.1 %) and Bio active Vermicompost tea (35%) but the chemical control it is heigh results in all treatment to control of pathogen only Potassium permanganate (69.3%), Ridomil Gold Plus(chemical fungicide) (66%) and Potassium phosphite (63.7%). However, Trichoderma and Actinomycetes recorded the best results in growth parameters (20.1 %) and (19.8), respectively in plant height followed by Bio active Vermi-wash 19.5 %, chitosan 19%, Bio active Vermicompost tea (16.1%), Potassium phosphite (16%), Potassium permanganate (15.7 %), Ridomil Gold Plus (15%) and control (11.3%). Inconclusion, this study has shown that the treatment with microbes derived from bioactive vermicompost is capable of combating Macrophomina phaseolina diseases while promoting plant growth, in accordance with the bio farming system that meets export standards.