Bovine brucellosis is considered as a major neglected zoonotic disease that are still present in animal populations of several developing countries, such as Egypt. These countries generally have less effective control programs and present high-density livestock, illuminating competition for limited resources between the implementation of control strategies and the economic consequences of those strategies. It is well known that bovine brucellosis can potentially cause public health problems and animal welfare issues. The present study is designed to investigate the use of GIS in determining the spatial distribution of bovine brucellosis in Menoufia governorate. The current study attempts to investigate the GIS and spatial distribution as an efficient in disease mapping worldwide. This review spot highlights on this important view about these approaches and its application on bovine brucellosis as a model example in Menoufia governorate.