The chamomile is a good source of phenolic and flavonoid compounds that act as a natural antioxidant. This study was designed to investigate the role of chamomile (CH) against gamma radiation (six gray) induced hepatotoxicity and renal toxicity. Liver markers, kidney function, lipid profile and oxidative stress Enzymes, i.e. MDA, CAT, SOD, and GSH were measured after radiation. The obtained results  showed that oral administration of chamomile extract (100mg /kg B.w) orally daily for four weeks  , significantly decrements in the  levels of transaminase enzyme in the serum of rats , as soon as significantly decrements in  the total lipids ,triglyceride and total cholesterol levels in serum of rats. Also improvement in kidney function through decrements in the creatinine and urea levels in serum comparing with irradiated group. Significant increments in the antioxidant enzymes (SOD and CAT) and GSH concentration. On the contrary, significant decrements in total free radical and MDA in blood and Liver tissues  comparing  with irradiated group. The present study suggests that chamomile extract has an antioxidant effect to radio protective effect. hence, chamomile is considered as source of natural antioxidant against gamma radiation effects.