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Molecular Investigation of blaTEM, sul1 and papG genes and Molecular Phylogenetic of Beta- lactamase TEM Gene in Multidrug Resistance Proteus mirabilis in Baquba city/ Iraq

Article

Last updated: 27 Apr 2025

OverviewSimilar Items

Subjects

-

Tags

Pathogenicity and virulence

Abstract

Background: Antimicrobial resistance in Proteus mirabilis is increasing worldwide, such as the resistance to sulfonamides and beta lactams due to the production of extended-spectrum β-lactamases (ESBL) in hospitalized patients. Objective: This study aimed to detect some antibiotic resistance genes of P. mirabilis bacteria causing various infections using PCR. Methodology: One hundred eighty specimens were collected from different clinical samples in Diyala. Antibiotics resistance and some virulence factors were detected. PCR was done for blaTEM, sul1 and papG genes and screened through uniplex PCR then sequencing and phylogenetic tree of blaTEM gene was established. Results: Twenty (18.18%) isolates of P. mirabilis were obtained. All P. mirabilis isolates were phenotypically positive (100%) for extracellular urease, swarming and biofilm formation. Antibiogram showed resistance towards Trimethoprim 95% followed by Ampicillin and Amoxicillin-Clavulanic acid 85% and 80% respectively. Ciprofloxacin and Gentamicin showed 35% resistance, while Levofloxacin and Amikacin showed 5% resistance. Ceftriaxone, Azithromycin and Cefixime showed 70%, 65% and 60% respectively. All isolates were sensitive to Meropenem. Isolates showed that 100% of isolates had sul1 gene, while papG gene was positive in 95% of isolates and finally blaTEM gene was found in 80% of isolates. blaTEM sequencing showed a similarity of 99% with the reference strains of P. mirabilis bacteria. Conclusion: P. mirabilis bacteria were resistant to several antibiotics, especially trimethoprim and ampicillin. Meropenem, followed by amikacin, was the most effective antibiotic against all isolates. The sul1 gene was the most common resistance gene, and the prevalence of blaTEM and papG genes was low.

DOI

10.21608/ejmm.2025.373610.1555

Keywords

P. mirabilis, blaTEM, sul1, papG, phylogenetic tree

Authors

First Name

Najaat

Last Name

Mohammed

MiddleName

A.

Affiliation

Biology Department, College of Education for Pure Science, University of Diyala

Email

pbio.najatahmed@uodiyala.edu.iq

City

-

Orcid

-

First Name

Saba

Last Name

Al-Zubaidi

MiddleName

J. J.

Affiliation

Biology Department, College of Education for Pure Science, University of Diyala

Email

saba.jasim@uodiyala.edu.iq

City

-

Orcid

-

Volume

34

Article Issue

4

Related Issue

54773

Issue Date

2025-10-01

Receive Date

2025-04-07

Publish Date

2025-10-01

Print ISSN

1110-2179

Online ISSN

2537-0979

Link

https://ejmm.journals.ekb.eg/article_424511.html

Detail API

http://journals.ekb.eg?_action=service&article_code=424511

Order

424,511

Type

New and original researches in the field of Microbiology.

Type Code

2,038

Publication Type

Journal

Publication Title

Egyptian Journal of Medical Microbiology

Publication Link

https://ejmm.journals.ekb.eg/

MainTitle

Molecular Investigation of blaTEM, sul1 and papG genes and Molecular Phylogenetic of Beta- lactamase TEM Gene in Multidrug Resistance Proteus mirabilis in Baquba city/ Iraq

Details

Type

Article

Created At

27 Apr 2025