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424202

Brain-Derived Neurotrophic Factor and Interleukin-1 Beta as Novel Biomarkers in Patients Infected With E.Coli O157:H7 And Entamoeba Histolytica

Article

Last updated: 27 Apr 2025

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Subjects

-

Tags

Clinical microbiology

Abstract

Background:  The immune response to E. coli O157:H7 and Entamoeba histolytica (E.histolytica) includes both humoral and cellular components; however, despite the immune response, these organisms are able to evade host defenses through production of anti-phagocytic factors and toxins which hinder immune cell functions, making resolution of disease more difficult. Objective: The aim was to study the immunological role and correlation of Brain-derived neurotrophic factor (BDNF) and Interleukin-1 beta (IL-1β) in patients with diarrhea due to E. coli O157:H7 and E. histolytica. Methodology: This study enrolled 60 healthy participants as a control group, 30 patients infected with E. histolytica, 30 patients infected with E. coli O157:H7 and E. histolytica were the case group. PCR was used to diagnose E. coli O157:H7. E. histolytica was diagnosed by using wet mount examination with 0.85% saline and Lugol's iodine. BDNF and IL-1β have been measured in patients' blood using the ELISA technique. Results: The patients who had E. histolytica infection or co-infections showed significantly heightened IL-1β concentrations above normal values and co-infected patients demonstrated the most extreme IL-1β response pattern. Patients infected with E. histolytica exhibited a positive yet insignificant correlation pattern between BDNF and IL-1β levels that also applied to patients with co-infections. Conclusion: Patients co-infected with E. coli O157:H7 and E. histolytica, as well as E. histolytica alone, had markedly elevated levels of IL-1β in the blood profile, whereas BDNF levels were unaffected by the intestinal infection.

DOI

10.21608/ejmm.2025.373913.1557

Keywords

E.coli O157:H7, E. histolytica, BDNF, IL-1β

Authors

First Name

Sinan

Last Name

Khayoon

MiddleName

Q.

Affiliation

University of Kufa, Faculty of Science, Department of Biology, Iraq

Email

sinanq.almiarij@uokufa.edu.iq

City

-

Orcid

-

First Name

Saleem

Last Name

Al-Hadraawy

MiddleName

K.

Affiliation

University of Kufa, Faculty of Science, Department of Biology, Iraq

Email

saleem.alhadrawi@uokufa.edu.iq

City

-

Orcid

-

First Name

ِAhmed

Last Name

Aljanaby

MiddleName

A.J.

Affiliation

University of Kufa, Faculty of Science, Department of Biology, Iraq

Email

ahmedjaloob@gmail.com

City

-

Orcid

-

Volume

34

Article Issue

4

Related Issue

54773

Issue Date

2025-10-01

Receive Date

2025-04-09

Publish Date

2025-10-01

Print ISSN

1110-2179

Online ISSN

2537-0979

Link

https://ejmm.journals.ekb.eg/article_424202.html

Detail API

http://journals.ekb.eg?_action=service&article_code=424202

Order

424,202

Type

New and original researches in the field of Microbiology.

Type Code

2,038

Publication Type

Journal

Publication Title

Egyptian Journal of Medical Microbiology

Publication Link

https://ejmm.journals.ekb.eg/

MainTitle

Brain-Derived Neurotrophic Factor and Interleukin-1 Beta as Novel Biomarkers in Patients Infected With E.Coli O157:H7 And Entamoeba Histolytica

Details

Type

Article

Created At

27 Apr 2025