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423051

Emergence of vanA and Sequencing in Vancomycin resistance Enterococcus faecalis Isolated from Urinary Tract Infections

Article

Last updated: 04 May 2025

OverviewSimilar Items

Subjects

-

Tags

Clinical microbiology

Abstract

Background: E. faecalis is a common microbe causing urinary tract infections in Najaf city, Iraq, having resistance to many antibiotics. Objectives: The present research aimed to identify vanA gene presence and sequence in E. faecalis for detection of vancomycin resistance. Methodology: From 140 specimens collected from UTI patients, we identefied 21 Enterococcus isolates using bacteriological tests, among them 15 (71.4%) were E. faecalis detected by using Vitek-2 system and molecular diagnosis using ddl gene of E. faecalis. The remainder isolates belong to other Enterococcus species. Results: In this study, total isolates were vancomycin resistant VRE using MIC technique. Molecular studies detected 12 (80%) of E. faecalis isolates which carry vanA gene. Also, the study showed that the reference isolate has three mutations and an identity 97-100% among vanA of the strains that include a number of Gram positive bacteria such as Staphylococcus aureus and Clostridium difficile, and other species of Enterococci. Conclusion: The incidence of vanA gene has developed great challenges, indicates that E. faecalis were reservoirs of vanA gene which may be transmitted to other bacteria especially Staphylococcus aureus and Clostridium difficile. It is significant for continued checking the spread of VRE and effective approaches that control and limit antibiotic resistance distribution.

DOI

10.21608/ejmm.2025.369359.1527

Keywords

DDL, Enterococcus faecalis, vanA, Sequence

Authors

First Name

Khitam

Last Name

Abbas

MiddleName

F.

Affiliation

Department of Microbiology, Faculty of Medicine, University of Kufa, Najaf

Email

khitamfabbas1@gmail.com

City

najaf

Orcid

-

First Name

Zahraa

Last Name

Motaweq

MiddleName

Y.

Affiliation

Faculty of Science, University of Kufa, Najaf

Email

zahraa.mutawak@uokufa.edu.iq

City

najaf

Orcid

-

Volume

34

Article Issue

4

Related Issue

54773

Issue Date

2025-10-01

Receive Date

2025-03-18

Publish Date

2025-10-01

Print ISSN

1110-2179

Online ISSN

2537-0979

Link

https://ejmm.journals.ekb.eg/article_423051.html

Detail API

http://journals.ekb.eg?_action=service&article_code=423051

Order

423,051

Type

New and original researches in the field of Microbiology.

Type Code

2,038

Publication Type

Journal

Publication Title

Egyptian Journal of Medical Microbiology

Publication Link

https://ejmm.journals.ekb.eg/

MainTitle

Emergence of vanA and Sequencing in Vancomycin resistance Enterococcus faecalis Isolated from Urinary Tract Infections

Details

Type

Article

Created At

27 Apr 2025