423413

The Role of Forkhead box protein O1 (FOXO-1) and Clinical Laboratory Investigations in Assessing the Severity of COVID-19

Article

Last updated: 11 May 2025

Subjects

-

Tags

Biochemistry
Clinical Biochemistry
Molecular Biology

Abstract

Background: The transmission of diseases across nations and continents is a significant disadvantage of globalization. In December 2019, COVID-19 emerged in Wuhan, China, as a new viral illness caused by a novel beta coronavirus. The forkhead box O1 (FoxO-1) transcription factors are reported to play a significant role in maintaining normal cell physiology by regulating survival, apoptosis, and oxidative stress. 
Objective: We aimed to detect the severity of COVID-19 by clinical laboratory investigation and epigenetic biomarkers FOXO-1.
Patients and methods: This study is a prospective case-control investigation involving 50 participants divided into two groups. Group I consists of 30 patients diagnosed with COVID-19. While Group II includes 20 healthy controls. The expression levels of FOXO-1 were evaluated using real-time quantitative polymerase chain reaction (RT-qPCR).  
Results: In comparison between COVID-19 patients and healthy individuals, we use many clinical laboratory investigations such as systolic and diastolic blood pressure, RBCs, hemoglobin, hematocrit, MCHC (g/dL), PLTs, WBCs, INR, albumin, ALT, AST, LDH, PT, creatinine, sodium (Na), random blood sugar (mg/dL), and FOXO-1. There are statistically significant differences in these parameters. The analysis of the ROC curve demonstrated, along with the AUC curve, notable diagnostic performance at a cut-off value of 0.34-fold, yielding an AUC of 0.874, with a sensitivity of 88.0% and a specificity of 95.0%.
Conclusion: Then conclude from these results that the expression levels of FOXO-1 and clinical laboratory investigations, including hematological, serological, and molecular tests, serve as biomarkers in determining the severity of COVID-19.

DOI

10.21608/ijabmb.2025.369090.1011

Keywords

Keywords: Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), Forkhead box protein O1 (FOXO-1), Coronavirus disease 2019 (COVID-19)

Authors

First Name

Medhat

Last Name

Elsayed

MiddleName

M

Affiliation

Biochemistry Division, Chemistry Department, Faculty of Science, Helwan University, Cairo, Egypt.

Email

medhat.mido93@yahoo.com

City

Cairo

Orcid

-

First Name

Hatem

Last Name

El-mezayen

MiddleName

A.

Affiliation

Biochemistry Division, Chemistry Department, Faculty of Science, Helwan University, Cairo, Egypt.

Email

hatem_mezayen@yahoo.com

City

-

Orcid

-

First Name

Olfat

Last Name

Shaker

MiddleName

G.

Affiliation

Medical Biochemistry and Molecular Biology Department, Faculty of Medicine, Cairo University, Giza, Egypt.

Email

olfatshaker@yahoo.com

City

-

Orcid

-

First Name

Yasmine

Last Name

Abdelfatah

MiddleName

-

Affiliation

Internal medicine Department, Faculty of Medicine, Cairo University, Giza, Egypt.

Email

yasmineabdelfatah@kasralainy.edu.eg

City

-

Orcid

-

First Name

Sara

Last Name

Abdo

MiddleName

M.

Affiliation

Biochemistry Division, Chemistry Department, Faculty of Science, Helwan University, Cairo, Egypt.

Email

sara.elamery@yahoo.com

City

-

Orcid

-

Volume

2

Article Issue

2

Related Issue

54622

Issue Date

2025-06-01

Receive Date

2025-03-22

Publish Date

2025-06-01

Page Start

39

Page End

54

Print ISSN

3009-7630

Online ISSN

3009-7991

Link

https://ijabmb.journals.ekb.eg/article_423413.html

Detail API

http://journals.ekb.eg?_action=service&article_code=423413

Order

423,413

Type

Original Article

Type Code

3,170

Publication Type

Journal

Publication Title

International Journal of Applied Biochemistry and Molecular Biology

Publication Link

https://ijabmb.journals.ekb.eg/

MainTitle

The Role of Forkhead box protein O1 (FOXO-1) and Clinical Laboratory Investigations in Assessing the Severity of COVID-19

Details

Type

Article

Created At

27 Apr 2025