The vannamei shrimp farming has several advantages, as this species is known for its disease resistance, short cultivation period, and relatively low feed conversion ratio (FCR). These benefits make vannamei shrimp highly desirable and one of the most commonly farmed shrimp species. However, challenges in shrimp farming still arise, particularly concerning declining environmental quality, which can lead to disease outbreaks. These diseases hinder the growth and development of the cultured shrimp. Nano cell application serves as an enzyme that enhances the efficiency of vitamins and enzymes in the shrimp's body. This study aimed to examine the effect of nano cell application on the water quality of the vannamei shrimp post-larvae culture. The research was conducted from May to June at the Marine Fish Hatchery Laboratory, Pangkep State Polytechnic of Agriculture, Pangkajene and Islands. The study used a completely randomized design (CRD) with four treatments and three replications:   A (0 ml dose), B (5 ml dose), C (10 ml dose), D (15 ml dose). The observed parameters included water quality indicators such as temperature, dissolved oxygen (DO), pH, salinity, ammonia, and nitrite levels. The results showed that the application of nano cells influenced water quality parameters, demonstrating its potential impact on maintaining optimal water conditions in the vannamei shrimp culture.