The transportation of live fish fry involves transferring the fry using specific treatments to ensure their survival during transit. This practice is important for maintaining the health and viability of the fry until they reach their intended destination. In general, fish fry are transported from one location to another to support distribution efforts while ensuring that the fry remain in stable and healthy condition throughout the journey. This study aimed to determine the effect of different oxygen concentrations on the survival of the goldfish fry (Carassius auratus), and to identify the oxygen concentration that yields the best survival outcome. A closed transportation system was used, with a transport duration of six hours. The fry used were the Ryukin goldfish fry, each with a total length of approximately 3cm and an average age of 60 days. The transportation containers consisted of plastic bags with a total volume of two liters, filled with one liter of water and different oxygen concentrations. Each bag contained three goldfish fry, and a total of 36 fry were used across four treatments, each replicated three times. The study included four treatment groups: Treatment A used an oxygen concentration of 3mg·L⁻¹, Treatment B used 4mg·L⁻¹, Treatment C used  mg·L⁻¹, and Treatment D (the control) had an oxygen concentration of 0mg·L⁻¹. All treatments were conducted over a six-hour period. The results indicated that oxygen concentration did not have a significant effect on the survival of goldfish fry. However, the oxygen concentrations of 3mg·L⁻¹, 4mg·L⁻¹, and 5mg·L⁻¹ all resulted in a 100% survival rate, suggesting that these concentrations are sufficient to maintain fry viability during short-term transportation.