The range of the nematode of the genus Contracaecum (Anisakidae) covers almost the entire water area of the World Ocean from the Arctic to the Antarctic. Within the range, the larvae of this nematode at the 3^rd stage of development are found in fish and invertebrates. The results of this research showed that the contracaecum 3^rd stage larvae were detected in the body cavity of Cyprinus carpio, Carassius gibelio,and Silurus glanis fish distributed in the water bodies of the Jizzakh and Khorezm regions of Uzbekistan. The results of the helminthological study prevalence of Contracaecum sp. larvae with Cyprinus carpio fish was 30.8, 25% with Carassius gibelio fish, and 22.2% with Silurus glanis fish. Regarding morphological characterictics, it was found that these larvae are close to the species Contracaecum quadripapillatum.  Molecular analysis confirmed that the ITS1-5.8S-ITS2 rDNA sequence exhibited 99.8% identity with C. quadripapillatum sequences available in GenBank based on BLAST comparison. To the best of our knowledge, the larvae of C. quadripapillatum has been recorded in Uzbekistan for the 1^st time.