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422935

MITIGATION OF HEAT STRESS EFFECT ON IN VITRO MATURED OOCYTES USING VITAMIN C IN GOAT

Article

Last updated: 04 May 2025

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Tags

Physiology

Abstract

The current investigation aimed to assess the effect of induced heat stress (40 and 42 ºC) on the maturation of goat oocyte in vitro. In addition to study the effect of vitamin C supplementation during IVM as a way to mitigate the heat stress effects. A total of 438 of oocyteswere recovered from goat ovaries using slicing technique. The oocytes were cultured in prewarmed (38.5, 40, and 42 ºC, according to the experimental group) IVM medium with or without vitamin C (VC), under mineral oil in CO2 incubator 5% CO2, with 95% humidity. Six treatment groups were established: T1 (control, 38.5°C, no vitamin C), T2 (38.5°C, 50 µg/mL vitamin C), T3 (40°C, 50 µg/mL vitamin C), T4 (40°C, no vitamin C), T5 (42°C, 50 µg/mL vitamin C), and T6 (42°C, no vitamin C). The results of cumulus expansion that were obtained in this study were as follows; 81.67, 90, 87.22, 72.67, 71.69, and 55.59% for T1, T2, T3, T4, T5, and T6 groups, respectively. The data of the first polar body extrusion were recorded as 25.66, 41.33, 31.11, 17.56, 25.86, and 10.55% for T1, T2, T3, T4, T5, and T6 groups, respectively. In conclusion, heat stress (42 ºC) significantly reduced goat oocyte's maturation in vitro, however, vitamin C supplementation (50 µg/mL) to IVM medium may improveoocyte maturation rate and/or reduced the negative effects of heat stress.

DOI

10.21608/ejap.2025.354537.1101

Keywords

Goat, oocyte, IVM, heat-stress, vitamin c

Authors

First Name

Hesham

Last Name

Shedeed

MiddleName

-

Affiliation

Animal and Poultry Production Division, Desert Research Center, Cairo 11753, Egypt.

Email

shedeed_2020@yahoo.com

City

-

Orcid

-

Volume

62

Article Issue

1

Related Issue

53779

Issue Date

2025-01-01

Receive Date

2025-01-21

Publish Date

2025-01-01

Page Start

55

Page End

60

Print ISSN

0302-4520

Online ISSN

2735-3028

Article File

EJAP_Volume 62_Issue 1_Pages 55-60.pdf

PDF . 493.2kB

Link

https://ejap.journals.ekb.eg/article_422935.html

Detail API

http://journals.ekb.eg?_action=service&article_code=422935

Order

6

Type

Original Article

Type Code

1,298

Publication Type

Journal

Publication Title

Egyptian Journal of Animal Production

Publication Link

https://ejap.journals.ekb.eg/

MainTitle

MITIGATION OF HEAT STRESS EFFECT ON IN VITRO MATURED OOCYTES USING VITAMIN C IN GOAT

Details

Type

Article

Created At

27 Apr 2025