A field experiment was conducted in this study, two dry beans of Faba bean seeds were obtained from Mallawy Agricultural Research Station, Minia, Egypt two varieties (Sakha-1 and Giza-843). During two seasons 2021/2022 and 2022/2023 to examine the impact of applying yeast extract and L-ascorbic acid on the chemical composition of two faba bean varieties (Sakha-1 and Giza-843). Foliar spray of yeast extract (YA: (25 and 50 ml/L) and L-ascorbic acid at rate (L-AA: 50 and 100 mg/L) either alone, or their combination and control. The impact of the preceding treatments on moisture content, total nitrogen, crude protein, crude lipids (CL), carbohydrates, total phenolic compounds, total flavonoids, total alkaloids (TAs), and phytic acid (PA) were assayed. Results showed that applying L-ascorbic acid and yeast extract to the foliage significantly enhances the chemical composition. Results indicated L-ascorbic acid was more effective than yeast extract. Total ash, nitrogen, crude protein (CP), crude lipids (CL), carbohydrates, total phenolic compounds, total flavonoids, total alkaloids (TAs), and phytic acid (PA) in seeds of faba bean were significantly enhanced by mounting L-ascorbic acid from 50 to 100 mg/L and/or yeast extract concentrations up to 25 and 50 ml/L. The qualitative screening of the bioactive secondary metabolites in the extracts of two varieties of broad bean included 12 groups and the recorded results indicate the presence of 10 groups i.e. phenolics, flavonoids, alkaloids, tannins, coumarins, glycosides, saponins, proanthocyanins, steriods and phytates and absence emadins and terpenoids. The concentrations of TPCs in all treatments are higher than the unsprayed samples (control). It is also clear that the concentrations of TPCs in the extracts of Sakha-1 cultivar are always higher than their counterparts in Giza-843 cultivar. It is clearly noted that the binary mixtures led to increases in TPCs more than the individual treatments. The treatment YA 50 mg/L + ASA, 100 mg/L is the best treatment as it recorded the highest concentration of TPCs in both cultivars. The concentrations of TFs in the two cultivars are close, and in some cases, the Sakha-1 cultivar has a higher concentration, unlike the concentrations of TPCs, which are higher in Sakha-1 cultivar in all treatments than in Giza-843 cultivar. The present results also indicate that TFs fluctuate within a narrow range, and the treatment YA 50 mg/ + ASA, 50 mg/L was the best treatment in the two cultivars, and the highest concentration of flavonoids was recorded at 88.37 for Sakha-1 cultivar and 88.44 for Giza-843 cultivar. Phytic acid concentrations were always higher in the extracts of Sakha-1 variety than in Giza-643 variety. The results also indicate that the treatment YA 50 mg/L + ASA, 100 mg/L achieved the highest concentration of phytic acid which plays an important role in resistance against bacteria, fungi and insects and is also a standard antioxidant.