Background: In Vitro Fertilizationis a widely used Assisted Reproductive Technology (ART) that helps individuals and couples overcome infertility by fertilizing an egg with sperm outside the body in a laboratory setting. The process involves stimulating the ovaries to produce multiple eggs, retrieving the eggs, and combining them with sperm in a controlled environment to create embryos. Aim of the study: This study aimed toevaluate the effect of self-care guidelines on knowledge and quality of life among women undergo In Vitro Fertilization. Research Design: A quasi-experimental research design (pre & posttest one group) was used. Setting: Obstetrics and gynecology department of the Maternal & Child Minia University Hospital's, high dependent unit (HDU1). Sample:A purposive sample of 65 women.Tools:Data was gathered using Four tools: A structured interview questionnaire, knowledge assessment tool, self-care guideline assessment tool, and Fertility Quality of Life (Ferti QoL) tool.   Results: The study showed that, 26.9% of the women had average knowledge, 79.6% of them had a satisfactory practice, and 78.5% a high quality of life after intervention regarding In Vitro Fertilization. Conclusion: This study concludes that implementing self-care guidelines lead to improve knowledge, practices and quality of life for women undergo in vitro fertilization. Recommendations: Apply educational program to womenundergo In Vitro Fertilization to increases knowledge, practices and quality of life in another health care settings.