Background: Ventilator-associated pneumonia (VAP) is the most common infectious complication among the patients admitted in the intensive care units (ICUs), which is developed in the patients receiving mechanical ventilation. Study aimed to assess Nurses' Knowledge, Attitude and Practice on Prevention of Ventilator-Associated Pneumonia (VAP) Among Critically Ill Patients in Omdurman Teaching Hospital. Methods. An exploratory hospital based study carried in Omdurman teaching hospital which include 45 nurses. Randomly selected by systemic random sampling. Data were collected via what's up and the questionnaire focused on knowledge attitude and practice regard (VAP), using descriptive (Mean+SD) for socio demographic data and inferential statistics for variable knowledge, attitude and practice Mean+SD ,and using chi squire and 0.001 as significant correlation and suggested that mean knowledge mean score  if >than 80%-75% consider good mean knowledge level and less than 75%-50% consider as moderate level,less than 50% considered poor . Same suggestion with attitude and preventive practice. Results: participants knowledge and practice were moderate, but the showed poor mean level attitude .there were significant relation between age, experience between knowledge, practice. Conclusion: participant showed moderate score level on knowledge and practice while poor level toward attitude. Recommendation: extensive educational programs should be arranged for nurses to improve their attitude regard ventilator patients in CCU so that can improve their attitude.