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423993

Cytotoxic activity of partial purified ExoT toxin produced by P. aeruginosa against MCF-7 and PC3 cancer cell lines

Article

Last updated: 27 Apr 2025

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Subjects

-

Tags

Clinical microbiology

Abstract

Background: ExotoxinT (ExoT) is one of these exotoxins secreted through the type three secretion system (T3SS) produced by Pseudomonas aeruginosa. Objectives:This study aims to investigate the ability of exotoxin A extracted from Pseudomonas aeruginosa to inhibit cancer cell lines breast cancer (MCF-7) and prostate cancer (PC3). Methodology: seventy one isolates were isolated from 242 clinical samples. The isolates were identified using various microscopic, biochemical, and cultural diagnostic methods.The toxin was then extracted and purified from the productive isolate. ExoT was purified using ammonium sulfate, ion exchange, and gel filtration chromatography (Sephadex G-150). The MTT test was performed to study its inhibitory effects against cancer cell lines in vitro. Results: The results of the MTT assay refer to half-maximal inhibition concentration (IC50) of partially purified ExotoxinT aganist MCF-7,PC3 and HDFn cells was 84.72, 193.1, and 243.9 μg/ml, respectively. The toxic effect was more significant on breast cancer cells than on prostate cancer cells for the values of IC50 compared to normal cells (HDFn). The effect of partially purified ExotoxinT on some criteria or indicators of apoptosis was evaluated by high-content screening that showed a significant effect when exposed to concentrations of 200, and 100 µg/ml. Conclusion: The effect of partially purified exotoxin T was studied by MTT assay and apoptosis indices by high-content assay and showed significant effects

DOI

10.21608/ejmm.2025.372841.1550

Keywords

Exotoxin T, P. aeruginosa, Cytotoxicity, cancer cell lines

Authors

First Name

Mohammed

Last Name

Ismaieel

MiddleName

S.

Affiliation

Department of Biology, College of Science, University of Anbar

Email

moh21s1010@uoanbar.edu.iq

City

ramadi

Orcid

-

First Name

Muthanna

Last Name

Hassan

MiddleName

H.

Affiliation

Department of Biology, College of Science, University of Anbar

Email

sc.muthanna-477-aliraqi@uoanbar.edu.iq

City

-

Orcid

0000-0002-8548-2146

First Name

May

Last Name

Flayyih

MiddleName

T.

Affiliation

Department of Biology, College of Science, University of Baghdad

Email

may.talib@sc.uobaghdad.edu.iq

City

baghdad

Orcid

-

Volume

34

Article Issue

2

Related Issue

51523

Issue Date

2025-04-01

Receive Date

2025-04-04

Publish Date

2025-04-01

Page Start

435

Page End

438

Print ISSN

1110-2179

Online ISSN

2537-0979

Article File

EJMM_Volume 34_Issue 2_Pages 435-438.pdf

PDF . 389.2kB

Link

https://ejmm.journals.ekb.eg/article_423993.html

Detail API

http://journals.ekb.eg?_action=service&article_code=423993

Order

423,993

Type

New and original researches in the field of Microbiology.

Type Code

2,038

Publication Type

Journal

Publication Title

Egyptian Journal of Medical Microbiology

Publication Link

https://ejmm.journals.ekb.eg/

MainTitle

Cytotoxic activity of partial purified ExoT toxin produced by P. aeruginosa against MCF-7 and PC3 cancer cell lines

Details

Type

Article

Created At

27 Apr 2025