In the present study, we report the simple, inexpensive, and eco-friendly synthesis of silver nanoparticles (AgNPs) to assess their potential in biomedical applications based on their particle size and stability. Therefore, two different AgNP samples were prepared using sunflower seed cake (SSC) and pomegranate peel (PP) aqueous extract. Different spectroscopic techniques were used to characterize the synthesized SSC-AgNPs and PP-AgNPs. UltraVilot-Visible (UV) Spectroscopy showed the characteristic peak around 450 nm which is caractristic to AgNPs . TEM analysis depicted the spherical morphology of SSC-AgNPs and PP-AgNPs with 4.46–10.16 nm and 14.11– 47.77 nm respectively. Dynamic light scattering (DLS) analysis showed that SSC-AgNPs and PP-AgNPs showed negative zeta potential value -9.79 and -24.22 mv respectively. The results of the XRD indicated that SSC- AgNPs and PP- AgNPs were in face cantered cube structure. Also, the results of Fourier transform infrared (FTIR) research showed that the SSC and PP extract contained proteins and polyphenols. Also, cytotoxicity studies using hemolytic and normal cell viability assays, as well as, MCF7 anticancer activity and the inhibition activity against three microbial strains of both AgNPs were investigated. The results showed that SSC-AgNPs and PP-AgNP were biologically compatible at concentration 2000 μg/mL and 250μg/mL while reduced cancer cell viability at concentration1878 µg/ml and 1140 µg/ml respectively. Therefore, SSC-AgNPs are better suited for biomedical applications than PP-AgNPs. It could be concluded that biosynthesis formation of AgNPs with different size and stability posse different impact on cytotoxicity and biological activity.